This is one of the more frequent questions I see as a genealogist specializing in lineage society applications. And the answer?
Maybe.
Remember that there are three societies that use the nickname “Colonial Dames.” (To learn more, visit our blog post on the topic.) Ancestors qualifying for the DAR would be too young to be qualifying for Colonial Dames XVII Century, which requires service before 1701. Ancestors who qualify for Colonial Dames of America might be of the right age to have served in the American Revolution, but the same service cannot be used. CDA requires service to have occurred on or before 18 April 1775. The eligibility requirements for National Society Colonial Dames of America overlap slightly with DAR, allowing service through 4 July 1776. You can search a roster of ancestors here. So, yes, some DAR paperwork will be acceptable for the Colonial Dames – but only for NSCDA, and only if service occurred before 4 July 1776.
But that paperwork needs to be documentation, not the application! NSCDA does not accept applications from other societies. You can only “reuse” the supporting documentation you found for this line in a new application. Citations and formatting must match that requested by the society.
Happy hunting!